Bio-oxidative treatments (Hydrogen Peroxide (H2O2) and Ozone (O3) treatments) have been used for many years and initially appeared in medical journals in 1920. Since then, studies have been conducted in many research centers around the world. First, the American researcher Dr. Charles H. Farr was nominated for the Nobel Prize in Medicine in 1993 for his research on Hydrogen Peroxide, which is essential for life and for the better functioning of our immune systems. Granulocytes, a type of white blood cell, produce H2O2, which is a defense mechanism against organisms such as bacteria, parasites and yeast that attack the body. H2O2 is also a hormone and blood sugar regulator, but is also known as a disinfectant, antiseptic and oxidant in the pharmaceutical and food industries. Therapeutically, it has been used successfully for circulatory disorders, lung diseases, parasitic infections, and other human disorders.

Ozone (O3) is an activated form of Oxygen with extra active electrons and initially was used as a disinfectant in World War I. Later, ozone was used as an antiseptic to kill bacteria, viruses and yeast. Ozone was also included in major research studies in Germany in 1930. It was used to help patients suffering from Crohn’s disease, colitis, and to destroy viruses such as hepatitis, shingles, megacytosis, and recently HIV. It has also been used as a regulator in chronic inflammation, diabetes mellitus, and in eye diseases and circulatory diseases. Nowadays, in the production of ozone for medical use, specialized devices are used that produce a range of O2 / O3 gas mixtures. Specific mix densities and quantities with defined protocols from the World Ozone Federation are used for each type of application.

H2O2 and O3 have significantly contributed to the treatment of many human diseases. As far as clinical doctors and researchers are concerned, they can also be effective with natural and inexpensive methods by applying modern knowledge of oxidative therapies to medical practice. Today, ozone research in medicine is being implemented in Germany, Cuba, Russia in cooperation with and with the support of governments and many universities. Also, research is less applied in the US, Italy, France, Egypt, India, Greece etc.

Ozone Properties and Medical Uses:
– Ozone has become an industry element of choice to disinfect drinking water in cities and to purify unsuitable water through a series of applications. It is also used in the food industry to suppress bacterial growth substrates in drinking water and processed foods.
– Over the last 60 years, more than 12 methods have been developed in the application of ozone therapy, where small amounts of O3 are added to pure O2 and a medical O2 / O3 mixture is produced from devices called medical ozonators.
– Methods for safe ozone therapy are: intramuscular injection, intra-articular infusion, small and large self-therapy, ozonated water application, ozone exposure methodology, bag treatment, ozone application externally and ozone depletion.

H2O2 is created in the atmosphere when ultraviolet radiation hits O3 in the presence of moisture. This is the reason why H2O2 is completely related to O3. It has been found in many therapeutic sources worldwide such as Portugal, France and Canada. It is an important factor in the life of plants and animals. Like ozone, it is administered under specific concentrations in intravenous serum, orally, in wound disinfection, baths, injections into the body, as injections into the joints, and into intramuscular injections of pain.

Today due to the easy titration and safe production of the medical O2 / O3 blend by medical ozonators when needed, the use of H2O2 has been restricted to external applications.


1. V. Bocci. “Ozone therapy today”. In Ozone in Medicine: Proceedings, 12th World Congress of International Ozone Association (Zurich 1993).

2. J. Varvo. “Ozone application in cancer cases”. In Medical application of Ozone, Edited by Iulius Laraus (Norwalk) (Pan-American committee 1983).

3. C. H. Farr. “The therapeutic use of H2O2” Townsend letter for Doctors. 1987.

4. Sylvia Menentez et al. “Ozone Therapy and Magnetotherapy” New methods for the rehabilitation of patients with simple chronic glaucoma” In Proceedings: Ozone in Medicine; 12th World Congress of International Ozone Association (Zurich 1995)

5. N. M. Robedinsky et al. “Effectives of O3 therapy in the treatment of condylomatosis in women” Ozone in Biology and Medicine. (Niz. Novgorod: Ministry of Public Health of Russia Federation, 1992.